Synthesis and application of structured triacylglycerol
Dissertation solver: MSc. Katsiaryna Alishevich
Supervisor: Filip Vladimír, prof. Ing. CSc. ( fil...@vscht.cz)
Berčíková Markéta. Ing. Ph.D.
Anotation: Structured triacylglycerols constitute crystal network most the fat blends of fat products. Microstructure of crystal network determines the nature of the non-bonded interactions with liquid triacylglycerols and thereby determines the physical and rheological properties of the final product.
The aim of this work will be the preparation of the pure triacylglycerols to study the properties of subsequently prepared fat blends. The characteristic behavior of triacylglycerols is polymorphic behavior which depends on the composition of TAG and heat treatment fat blends. Prepared structured triacylglycerols and fat blends will be assessed by physical methods used to study crystallization, microstructure and rheological properties.
Synthesis, functional properties and stability of lipid nanoparticles
Dissertation solver: MSc. Radek Beneš
Supervisor: Kyselka Jan, doc. Ing. CSc. (jan.kyselka@vscht.cz)
Annotation: Lipid nanoparticles are successfully used in clinical practice as they are safe and efficient delivery systems that protect drugs or nucleic acids from degradation. An important application of the lipid nanoparticle-mRNA system is the vaccines against the coronavirus disease COVID-19. From the point of view of good manufacturing practice, the stability and safety of both the lipids and the prepared nanoparticles are crucial. Lipids are usually purposefully modified and represent a complex system composed of cationic (DOTMA, DOSPA, DOTAP), amphoteric (phospholipids) and nonionic (ALC-0159, PEG2000-DMG) tensides, sterols or their derivatives (DC-cholesterol, BHEM-cholesterol). The aim of the work is the laboratory preparation of modified lipids and sterols from the group of cationic, amphoteric and non-ionic tensides as well as description of their functional properties. Furthermore, the prepared lipid nanoparticles and their stability under defined conditions will be characterized.
Fractionation of prebiotic galactooligosaccharides and their application properties
Supervisor: Čurda Ladislav, doc. Ing. CSc. ( cur...@vscht.cz)
Characteristics and functional properties of sourdough with lactobacilli
Supervisor: Plocková Milada, doc. Ing. CSc. ( plo...@vscht.cz)
Horáčková Šárka, Ing. CSc. ( sar...@vscht.cz)
The aim of the thesis will be preparation and comparison of the sourdoughs from various cereals and pseudocereals and their analytical and nutritional assessment. Attention will be paid to the presence of particular groups of microorganisms focusing on the genus Lactobacillus, the characterization of newly isolated strains with emphasis on their antibacterial and antifungal activities. Furthmore, the effect of present microflora on the quality of prepared sourdoughs, dough and bakery products, especially on the changes of polysaccharides and products aging with additions of various kinds of sourdoughs will also be evaluated.
Stabilization and modification of food additives and nutraceuticals by catalytic hydrogenation
Supervisor: Filip Vladimír, prof. Ing. CSc. ( fil...@vscht.cz)
Kyselka Jan, Ing. Ph.D. ( KYS...@vscht.cz)
The effect of the modification of milk protein by microbial transglutaminase on the functional properties of milk.
Supervisor: Štětina Jiří, doc. Ing. CSc. ( ste...@vscht.cz)
Čurda Ladislav, doc. Ing. CSc. ( cur...@vscht.cz)
Synthesis of new type of natural antioxidants and their acetals for industrial frying and deep-frying applications.
Dissertation solver: MSc. Petra Šimicová
Supervisor: Kyselka Jan, Ing. Ph.D. ( KYS...@vscht.cz)
Hrádková Iveta, Ing. Ph.D. ( ive...@vscht.cz)
Filip Vladimír, prof. Ing. CSc. ( fil...@vscht.cz)
Anotation: The thermal and oxidative stability of oils and fats are essential criteria in the selection of frying media for industrial applications. The development of products with minimal trans-unsaturated fatty acid content and reduced saturated fatty acid content is actual. The use of synthetic antioxidants is also on the decline and therefore the main challenge is to find a suitable frying medium with respect to the fatty acid composition and its subsequent fortification with natural antioxidants or their concentrates. An interesting alternative is the application of hydrophobic lignans contained in sesame oil, in particular sesamolin, as it provides an effective natural antioxidant, sesamol, when hydrolyzing the acetal linkage in situ. The aim of this work will be the organic synthesis of new type of natural antioxidants including their acetals for industrial frying and deep-frying applications, which will protect unsaturated fatty acids from autooxidation and thus prolong the durability of the frying medium. The efficacy of the newly prepared compounds will be compared with synthetic antioxidants or commercial concentrates of lignans of Sesamum indicum.
Study of the influence of antimicrobial substances on the stability of emulsion systems
Dissertation solver: MSc. Mariana Dančíková
Supervisor: Hrádková Iveta, Ing. Ph.D. ( ive...@vscht.cz)
Anotation: The use of antimicrobial substances in cosmetic products is a frequently discussed topic in the connection with the use of the new substances, their toxicological profile and the influence on physical, chemical and microbiological stability of products.
The aim of thesis is the design of model cosmetic systems; the comparison of antimicrobial effect of selected antimicrobial compounds (commercial and newly synthesized) in model systems; the determination of skin penetration and potential for skin irritation of tested antimicrobial substances in different model systems by alternative toxicological method in vivo in system of reconstructed 3D models of human skin; the monitoring of physical-chemical stability of model cosmetic systems and formation of nitrosamines, formaldehyde and other toxic substance in dependence on chemical structure of biological active substances in model system.
Dependence of quaternary ammonium salts antimicrobial effect on their structure
Dissertation solver: MSc. Kateřina Sasínová
Supervisor: Šmidrkal Jan, prof. Ing. CSc. ( jan...@vscht.cz)
Berčíková Markéta. Ing. Ph.D.
Anotation: Some of quaternary ammonium salts are used in the form of pure substance as disinfection substance in human and veterinary medicine (Ajatin, Septonex). Other quaternary ammonium salts in the form of technical mixture are used as surfactants with the antimicrobial and antistatic effect in the skin cleaning cosmetics. The antimicrobial effect can be enhanced by altering the structure of the molecule of substance.
In the theoretical part, find the quaternary ammonium salt in the literature, which are used as antimicrobial substances in medicine and cosmetics. Evaluate the literature data and suggest the synthesis of new quaternary ammonium salts, which would have higher antimicrobial effect.
In the experimental part of the work, synthesize several series of pure quaternary ammonium salts. Optimize their synthesis conditions. Determine their critical micellar concentration and antimicrobial effect and compare it with the effect of currently used quaternary salts.