Assoc. Prof. Jan Kyselka, MSc, PhD

Working group: Fats, Surfactants, Cosmetics

Pedagogical activity:

• Courses for BSc. degree:
  • Food technology (part fat technology)
  • Food technology and biotechnology (part fat technology)

• Courses for MSc. degree:
  
  • Proces food chemistry 
  • Laboratory of food technology and process control
  • Technological project
• Supervision of bachelor's and diploma theses, supervision of student projects.
•  Supervision of disertation thesis

Scientific activity:

• Isolation and analysis of lipid classes (homo-, heterolipids)
• Synthesis of selected oxidation products (sterols, fatty acids, tocopherols)
• The extraction and refining of vegetable oils
• 3-MCPD diesters, glycidol monoestres – mitigation stratégy

 Education:

• 2005 – 2010 MSc., ICT Prague, Dairy and Fat Technology
• 2010 – 2014 PGS, ICT Prague, Food Technology
• 2021 Associate Professor, Food Technology

 Employment experiences:

• 2012 – Present, ICT Prague, Department of Dairy, Fat and Cosmetisc
• 2024 Vice-Dean for Science and Research

 Fellowships

• 2012 University of Alberta, Edmonton, Canada

 Selected scientific papers since 2015:

 ORCID ID 0000-0003-4071-0480

• Kalenchak, K., Novakova, L., Vachalova, T., Honzikova, T., Hybner, T., Rajchl, A., . . . Kyselka, J. (2026). New Insight into Cavitation-Assisted Chemical Refining and Enzymatic Modification of Vegetable Oils and Their Impact on Physicochemical Properties of Final Products. FOODS, 15(3). doi:10.3390/foods15030439
• Bártová, Veronika, Jan Bárta, Markéta Jarošová, Jan Bedrníček, František Lorenc, Adéla Stupková, Josef Švajner, Pavel Smetana, Jan Kyselka, Vladimír Filip, Zbyněk Zdráhal, and Marie Bjelková. "Milk Thistle (Silybum Marianum L. Gaertner) Oilseed Cake Flour Functional, Nutritional and Antioxidant Characteristics as Effect of Cultivar and Preparation Process." Food Bioscience 63 (2025). https://dx.doi.org/10.1016/j.fbio.2024.105735.
• Benes, R., D. Koval, I. Svec, A. Macurkova, B. Vrchotova, T. Honzikova, K. Kalenchak, J. Barta, V. Bartova, J. Bedrnicek, F. Lorenc, P. Smetana, and J. Kyselka. "Antimicrobial and Antifungal Activities of Proline-Based 2,5-Diketopiperazines Occurring in Food and Beverages and Their Synergism with Lactic Acid." ACS Agric Sci Technol 5, no. 8 (Aug 18 2025): 1681-92. https://dx.doi.org/10.1021/acsagscitech.5c00236.
• Kalenchak, Katsiaryna, Markéta Berčíková, Kateřina Sasínová, Tereza Honzíková, Radek Beneš, Jan Rohlíček, Jan Kyselka, and Vladimír Filip. "Ternary Phase Behavior of 1,3-Dipalmitoyl-2-Linoleoylglycerol (Plp), 1-Palmitoyl-2-Linoleoyl-3-Stearoyl-Rac-Glycerol (Pls), and 1,3-Distearoyl-2-Linoleoylglycerol (Sls): Minor Tag Species of Vegetable Fats and Oils." Crystal Growth & Design 25, no. 5 (2025): 1372-85. https://dx.doi.org/10.1021/acs.cgd.4c01308.
• Tomas Kourimsky, Jakub Tomasko, Beverly Hradecka, Vojtech Hrbek, Jan Kyselka, Jana Pulkrabova, Jana Hajslova: Chlorinated paraffins as chlorine donors for the formation of 2- and 3-chloropropanediols in refined vegetable oils. Food Chemistry 465, 141919, 11 pages (2025) https://dx.doi.org/10.1016/j.foodchem.2024.141919
• Jarošová M., Lorenc F., Bedrníček J., Petrášková E., Bjelková M., Bártová V., Jarošová E., Zdráhal Z., Kyselka J., Smetana P., Kadlec J., Stupková A., Bárta J. (2024). Comparison of Yield Characteristics, Chemical Composition, Lignans Content and Antioxidant Potential of Experimentally Grown Six Linseed (Linum usitatissimum L.) Cultivars. Plant Foods for Human Nutrition 79, 159–165. DOI: 10.1007/s11130-023-01136-9
• Jarošová M., Roudnický P., Bárta J., Zdráhal Z., Bártová V., Stupková A., Lorenc F., Bjelková M., Kyselka J., Jarošová E., Bedrníček J., Bohatá A. (2024). Proteomic Profile of Flaxseed (Linum usitatissimum L.) Products as Influenced by Protein Concentration Method and Cultivar. Foods, 13(9), 1288. https://doi.org/10.3390/foods13091288
• Alishevich, Katsiaryna, Markéta Berčíková, Jan Kyselka, Kateřina Sasínová, Tereza Honzíková, Petra Šimicová, Jan Šmidrkal, Jan Rohlíček, and Vladimír Filip. "Binary Phase Behavior of 2-Oleoyl-1-Palmitoyl-3-Stearoyl-Rac-Glycerol (Pos) and 2-Linoleoyl-1-Palmitoyl-3-Stearoyl-Rac-Glycerol (Pls)." Food Biophysics 18, no. 1 (2023): 161-73. https://dx.doi.org/10.1007/s11483-022-09761-8.
• Barta, J., P. Roudnicky, M. Jarosova, Z. Zdrahal, A. Stupkova, V. Bartova, Z. Krejcova, J. Kyselka, V. Filip, V. Riha, F. Lorenc, J. Bedrnicek, and P. Smetana. "Proteomic Profiles of Whole Seeds, Hulls, and Dehulled Seeds of Two Industrial Hemp (Cannabis Sativa L.) Cultivars." Plants (Basel) 13, no. 1 (Dec 30 2023). https://dx.doi.org/10.3390/plants13010111.
• Honzikova, T., M. P. Agbaga, R. E. Anderson, R. Brush, M. Ahmad, L. Musilkova, K. Sejstalova, K. Alishevich, R. Benes, P. Simicova, M. Bercikova, V. Filip, and J. Kyselka. "Novel Approaches for Elongation of Fish Oils into Very-Long-Chain Polyunsaturated Fatty Acids and Their Enzymatic Interesterification into Glycerolipids." J Agric Food Chem 71, no. 46 (Nov 22 2023): 17909-23. https://dx.doi.org/10.1021/acs.jafc.3c05355.
• Stellner, N. I., Z. S. Rerop, J. Kyselka, K. Alishevich, R. Benes, V. Filip, G. Celik, M. Haack, M. Ringel, M. Masri, and T. Bruck. "Value-Added Squalene in Single-Cell Oil Produced with Cutaneotrichosporon Oleaginosus for Food Applications." J Agric Food Chem 71, no. 22 (Jun 7 2023): 8540-50. https://dx.doi.org/10.1021/acs.jafc.3c01703.
• Dragoun, M., Klausova, K., Simicova, P., Honzikova, T., Stejskal, J., Navratilova, K.,  Hajslova, J., Barta, J., Bartova, V., Jarosova, M., Bjelkova, M., Filip, V., Kyselka, J. (2022). Formation of Previously Undescribed Delta(7)-Phytosterol Oxidation Products and Tocopherylquinone Adducts in Pumpkin Seed Oil during Roasting, Screw-Pressing, and Simulated Culinary Processing at Elevated Temperatures. J Agric Food Chem, 70(37), 11689-11703. doi:10.1021/acs.jafc.2c03292
• Bárta, Jan, Veronika Bártová, Markéta Jarošová, Josef Švajner, Pavel Smetana, Jaromír Kadlec, Vladimír Filip, Jan Kyselka, Markéta Berčíková, Zbyněk Zdráhal, Marie Bjelková, and Marcin Kozak. 2021. “Oilseed Cake Flour Composition, Functional Properties and Antioxidant Potential as Effects of Sieving and Species Differences.” Foods 10(11). https://doi.10.3390/foods10112766.
• Belkova, B., L. Chytilova, V. Kocourek, M. Slukova, K. Mastovska, J. Kyselka, and J. Hajslova. 2021. “Influence of Dough Composition on the Formation of Processing Contaminants in Yeast-Leavened Wheat Toasted Bread.” FOOD CHEMISTRY 338. doi: 10.1016/j.foodchem.2020.127715 
• Brychova, Veronika, Mélanie Roffet-Salque, Ivan Pavlu, Jan Kyselka, Pavlina Kyjakova, Vladimir Filip, Svetlik Ivo, and Richard P. Evershed. 2021. “Animal Exploitation and Pottery Use during the Early LBK Phases of the Neolithic Site of Bylany (Czech Republic) Tracked through Lipid Residue Analysis.” Quaternary International 574:91–101. https://doi.10.1016/j.quaint.2020.10.045 .
• Koval, Daniel, Katsiaryna Alishevich, Kateřina Sasínová, Adéla Ramešová, Štěpán Marhons, Tereza Nešporová, Ladislav Čurda, Monika Kumherová, Jan Bárta, Vladimír Filip, and Jan Kyselka. 2021. “Formation of Dihydrophenolic Acids and Aroma-Active Volatile Phenols by New Strains of Limosilactobacillus Fermentum.” European Food Research and Technology 248(2):599–611. https://doi.10.1007/s00217-021-03907-7 .
• Kyselka, Jan, Klára Cihelková, Daise Lopes-Lutz, Josef Chudoba, Tereza Váchalová, Katsiaryna Alishevich, Iveta Hrádková, Markéta Berčíková, Monika Mikolášková, and Vladimír Filip. 2021. “Mechanism Controlling High-Temperature Degradation of Sunflower Oil Triacylglycerols in the Absence of Oxygen.” European Journal of Lipid Science and Technology 123(3). doi: 10.1002/ejlt.202000228.
• Berčíková, Markéta, Anežka Šimková, Klára Hudecová, Jan Kyselka, Vladimír Filip, and Iveta Hrádková. 2020. “Physical Properties of Structured Fats and Fat Blends Based on the Long Chain Fatty Acids.” Food Biophysics 15(1):143–52. doi: 10.1007/s11483-019-09609-8.
• Feldeková, Eva, Kateřina Solichová, Šárka Horáčková, Monika Kumherová, and Jan Kyselka. 2020. “The Impact of L-Lanthionine Supplementation on the Production of Nisin by Lactococci.” European Food Research and Technology 246(4):845–51. doi: 10.1007/s00217-020-03449-4.
• Koval, Daniel, Milada Plocková, Jan Kyselka, Pavel Skřivan, Marcela Sluková, and Šárka Horáčková. 2020. “Buckwheat Secondary Metabolites: Potential Antifungal Agents.” Journal of Agricultural and Food Chemistry 68(42):11631–43. doi: 10.1021/acs.jafc.0c04538.
• Kyselka, Jan, Tereza Honzíková, Tereza Váchalová, Tereza Jirásková, Katsiaryna Alishevich, Zdeňka Rottnerová, Veronika Brychová, Markéta Berčíková, Iveta Hrádková, and Vladimír Filip. 2019. “Formation of 5α-Sitostan-3-One, 5α-Campestan-3-One, and Steroidal Hydrocarbons in Edible Oils during Catalytic Hydrogenation.” Journal of Agricultural and Food Chemistry 67(35):9916–25. doi: 10.1021/acs.jafc.9b03054.
• Bialasová, Kristina, Irena Němečková, Jan Kyselka, Jiří Štětina, Kateřina Solichová, and Šárka Horáčková. 2018. “Influence of Flaxseed Components on Fermented Dairy Product Properties.” Czech Journal of Food Sciences 36(1):51–56. doi: 10.17221/411/2017-CJFS.
• Kyselka, Jan, Kateřina Matějková, Jan Šmidrkal, Markéta Berčíková, Erik Pešek, Beverly Bělková, Vojtěch Ilko, Marek Doležal, and Vladimír Filip. 2018. “Elimination of 3-MCPD Fatty Acid Esters and Glycidyl Esters during Palm Oil Hydrogenation and Wet Fractionation.” European Food Research and Technology 244(11):1887–95. doi: 10.1007/s00217-018-3101-9.
• Kyselka, Jan, Roman Bleha, Miroslav Dragoun, Kristýna Bialasová, Šárka Horáčková, Martin Schätz, Marcela Sluková, Vladimír Filip, and Andriy Synytsya. 2018. “Antifungal Polyamides of Hydroxycinnamic Acids from Sunflower Bee Pollen.” Journal of Agricultural and Food Chemistry 66(42):11018–26. doi: 10.1021/acs.jafc.8b03976.
• Szydłowska-Czerniak, Aleksandra, Dobrochna Rabiej, Jan Kyselka, Miroslav Dragoun, and Vladimír Filip. 2018. “Antioxidative Effect of Phenolic Acids Octyl Esters on Rapeseed Oil Stability.” Lwt 96:193–98. doi: 10.1016/j.lwt.2018.05.033.
• Kreps, František, Zuzana Burčová, Michal Jablonský, Aleš Ház, Vladimír Frecer, Jan Kyselka, Štefan Schmidt, Igor Šurina, and Vladimír Filip. 2017. “Bioresource of Antioxidant and Potential Medicinal Compounds from Waste Biomass of Spruce.” ACS Sustainable Chemistry and Engineering 5(9):8161–70. doi: 10.1021/acssuschemeng.7b01816.
• Kreps, František, Jan Kyselka, Zuzana Burčová, Štefan Schmidt, Aleš Rajchl, Vladimír Filip, Aleš Ház, Michal Jablonský, Alexandra Sládková, and Igor Šurina. 2017. “Influence of Deodorization Temperature on Formation of Tocopherol Esters and Fatty Acids Polymers in Vegetable Oil.” European Journal of Lipid Science and Technology 119(3). doi: 10.1002/ejlt.201600027.
• Kyselka, Jan, Dobrochna Rabiej, Miroslav Dragoun, František Kreps, Zuzana Burčová, Irena Němečková, Jana Smolová, Marie Bjelková, Aleksandra Szydłowska-Czerniak, Štefan Schmidt, Luděk Šarman, and Vladimír Filip. 2017. “Antioxidant and Antimicrobial Activity of Linseed Lignans and Phenolic Acids.” European Food Research and Technology 243(9):1633–44. doi: 10.1007/s00217-017-2871-9.
• Matlova, Veronika, Mélanie Roffet-Salque, Ivan Pavlu, Jan Kyselka, Ivona Sedlarova, Vladimir Filip, and Richard P. Evershed. 2017. “Defining Pottery Use and Animal Management at the Neolithic Site of Bylany (Czech Republic).” Journal of Archaeological Science: Reports 14:262–74. doi: 10.1016/j.jasrep.2017.05.028.
• Smolová, Jana, Irena Němečková, Marcela Klimešová, Zdeněk Švandrlík, Marie Bjelková, Vladimír Filip, and Jan Kyselka. 2017. “Flaxseed Varieties: Composition and Influence on the Growth of Probiotic Microorganisms in Milk.” Czech Journal of Food Sciences 35(1):18–23. doi: 10.17221/356/2016-CJFS.
• Kreps, František, Jan Kyselka, Zuzana Burčová, Štefan Schmidt, Vladimír Filip, Tibor Dubaj, Peter Gajdoš, and Milan Čertík. 2016. “Synthesis and Analysis of Tocopheryl Quinone and Tocopherol Esters with Fatty Acids in Heated Sunflower Oil.” European Journal of Lipid Science and Technology 118(5):788–802. doi: 10.1002/ejlt.201500218.
• Kyselka, Jan, Lukas Thomes, Simona Remisova, Miroslav Dragoun, Marketa Bercikova, and Vladimir Filip. 2016. “Preparation of Conjugated Linoleic Acid Enriched Derivatives by Conventional and Biphasic Isomerisation.” Czech Journal of Food Sciences 34(6):511–21. doi: 10.17221/362/2016-CJFS.
• Filip, Vladimir, Jan Kyselka, Iveta Hrádková, Markéta Berčíková, and Klára Cihelková. 2015. “A Versatile Apparatus for the Bench Scale Bleaching and Deodorisation of Vegetable Oils.” Czech Journal of Food Sciences 33(6):537–44. doi: 10.17221/438/2015-CJFS.
• Kyselka, Jan, Frantisek Kreps, Veronika Matlova, Lucie Pustelnikova, Pavel Ulbrich, Jiri Stetina, Stefan Schmidt, and Vladimir Filip. 2015. “The Effect of Rapeseed Microstructure on the Mechanism of Solution and Diffusion Extraction.” European Food Research and Technology 240(4):853–63. doi: 10.1007/s00217-014-2392-8.
• Kyselka, Jan, Frantisek Kreps, Volodymyr Skalka, Veronika Matlova, Lucie Pustelnikova, Jaroslav Kolacek, Jiri Stetina, Stefan Schmidt, and Vladimir Filip. 2015. “About the Origin of Asclepic Acid Derived from Crude Homo- and Heterolipids during Successive Solvent Extraction of Rapeseeds.” European Food Research and Technology 240(3):477–87. doi: 10.1007/s00217-014-2346-1.
• Kyselka, Jan, Frantisek Kreps, Veronika Matlova, Lucie Pustelnikova, Pavel Ulbrich, Jiri Stetina, Stefan Schmidt, and Vladimir Filip. 2015. “The Effect of Rapeseed Microstructure on the Mechanism of Solution and Diffusion Extraction.” European Food Research and Technology 240(4):853–63. doi: 10.1007/s00217-014-2392-8.